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bsa anti s6k  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank bsa anti s6k
    Bsa Anti S6k, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+anti+s6k/anti-Osteonectin/pmc11031405-406-34-45
    Average 93 stars, based on 20 article reviews
    bsa anti s6k - by Bioz Stars, 2026-09
    93/100 stars

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    Blocking Assay:

    Article Title: Inhibition of S6K lowers age-related inflammation and increases lifespan through the endolysosomal system
    Article Snippet: Ten micrograms (fly tissue and mouse tissue cell fractions) or 25 μg (mouse whole tissue lysis) of protein per lane on Any kD or 4–20% Criterion TGX stain-free precast gels (Bio-Rad) was transferred to Immobilon-FL polyvinylidene fluoride membrane (Millipore, #IPFL00010). .. Membranes were blocked by Intercept TBS Blocking Buffer (LI-COR, #927-60001) or 5% BSA for 1 h and probed with the following primary antibodies diluted in Intercept T20 TBS Antibody Diluent (LI-COR, #927-65001) or 5% BSA: anti-S6K (1:1,000), anti-pS6K T398 (Cell Signaling Technology, #9209, 1:1,000), anti-Relish (Developmental Studies Hybridoma Bank, #21F3, 1:100), anti-tubulin (Sigma, #T9026,1:5,000), anti-puromycin (Sigma, #MABE343, 1:1,000), anti-Stx12 (Synaptic Systems, #110 132, 1:1,000), anti-RelA (Cell Signaling Technology, #8242, 1:1,000), anti-RelB (Cell Signaling Technology, #4922, 1:1,000), anti-NF-κB2 (Cell Signaling Technology, #4882, 1:1,000), anti-GAPDH (Cell Signaling Technology, #2118, 1:5,000), anti-Histone H3 (abcam, #ab1791, 1:10,000). .. The following secondary antibodies were used: IRDye 800CW Goat anti-Mouse IgG (H + L) (LI-COR, #926-32210, 1:15,000), IRDye 680RD Goat anti-Rabbit IgG (H + L) (LI-COR, #926-68071, 1:15,000), HRP-conjugate Goat anti-Rabbit IgG Antibody (Sigma, #12-348; 1:10,000) and HRP-conjugate Goat anti-Mouse IgG Antibody (Sigma, #12-349; 1:10,000).



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    93
    Developmental Studies Hybridoma Bank bsa anti s6k
    Bsa Anti S6k, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+anti+s6k/anti-Osteonectin/pmc11031405-406-34-45
    Average 93 stars, based on 1 article reviews
    bsa anti s6k - by Bioz Stars, 2026-09
    93/100 stars
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